Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Role of Isoleucine in protein structure.

  • Please log in to reply
1 reply to this topic

#1 le_duy211



  • Active Members
  • Pip
  • 12 posts

Posted 17 September 2012 - 10:23 PM

What is the role of isoleucine in protein structure?. I found that many recombinant proteins have substituted threonine by isoleucine. I don't know the goal of this substitution.

#2 ascacioc



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 271 posts

Posted 19 September 2012 - 01:00 PM

The only reason I see it would be that Ile is bulkier and more hydrophobic vs Thr which is smaller and has a hydroxyl that can make hydrogen bonds so Ile would block pockets/make pocket smaller and would disrupt hydrogen bond formation...but these are speculations based on the structural knowledge. I do not have exact explanation why Thr-->Ile. Except that on the codon level you change a C in a T and you get easily from Thr to Ile and these C-->T transitions are very easy to happen.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.