Buffer in Gel
Posted 17 September 2012 - 04:00 AM
How often do you change the buffer in your gel electrophoresis. I am talking about the buffer in the gel tray.
And how often should it be changed (recommended)?
Posted 17 September 2012 - 05:05 AM
genius does what it must
i do what i get paid to do
Posted 17 September 2012 - 10:23 PM
Posted 17 September 2012 - 11:28 PM
If I'm just running a quick gel to check a PCR or something, I'd reuse the buffer in the tank for a second or third time.
Posted 18 September 2012 - 05:53 AM
Posted 18 September 2012 - 11:34 PM
Posted 19 September 2012 - 11:53 AM
If there was or is going to be a longer run, use fresh buffer. You can tell the condition of your buffer by the amount of heat the gel produces, if it goes warm it's not very good. But it still may not affect the results much.
I don't run elfo regulary so I reuse only when I do a set of electrophoresis, I didn't manage to force people to label the tanks with date the buffer was used last time and number of times it was used, so when I'm doing elfo after some tome and come to the tank with buffer I rather change it, because it may be off.
I wouldn't reuse TAE, it's bad as it is already.
I never trust anything that can't be doubted.