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alpha synuclein expression and purification

protein purification alpha syn aggregation troubleshooting

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#1 salema



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Posted 16 September 2012 - 12:28 AM

I have just recently expressed and purified alpha synuclein from BL-21 DE3 cells. The issue is, my protein won't aggregate even after 5 days of shaking at 37C. Now I can't think of reasons why this is happening. My Th-S counts have abrely changed in the last 5 days. It is slightly bizarre and we have never had this problem before. Has anyone had similar problems and what have you done to solve this?

Also tagged with one or more of these keywords: protein purification, alpha syn, aggregation, troubleshooting

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