Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

alpha synuclein expression and purification

protein purification alpha syn aggregation troubleshooting

  • Please log in to reply
No replies to this topic

#1 salema

salema

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 16 September 2012 - 12:28 AM

I have just recently expressed and purified alpha synuclein from BL-21 DE3 cells. The issue is, my protein won't aggregate even after 5 days of shaking at 37C. Now I can't think of reasons why this is happening. My Th-S counts have abrely changed in the last 5 days. It is slightly bizarre and we have never had this problem before. Has anyone had similar problems and what have you done to solve this?





Also tagged with one or more of these keywords: protein purification, alpha syn, aggregation, troubleshooting

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.