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alpha synuclein expression and purification

protein purification alpha syn aggregation troubleshooting

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#1 salema

salema

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Posted 16 September 2012 - 12:28 AM

I have just recently expressed and purified alpha synuclein from BL-21 DE3 cells. The issue is, my protein won't aggregate even after 5 days of shaking at 37C. Now I can't think of reasons why this is happening. My Th-S counts have abrely changed in the last 5 days. It is slightly bizarre and we have never had this problem before. Has anyone had similar problems and what have you done to solve this?





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