I'm new to serum sample ELISA and if anyone can kindly help me, I'd really appreicate it.
1. Can I use DuoSet from R&D to detect IFN-g and IL-4 in mouse serum? Or do I have to use specific kits that are designed for serum samples?
2. If specific serum kits are needed, do you have any recommendations? Anyone has experience with ebioscience products?
3. I'm wondering whether I have to dilute my serum sample. The cytokine level might be low so that I can't dilute it. If not diluted, the serum matrix is very complex and different from that of the reagent diluent used to dilute the standards. Are these two background comparable?
Thanks in advance
Edited by dreamerbac, 14 September 2012 - 07:25 PM.