Which is a better way to culture cells after thawing ?
Posted 14 September 2012 - 03:35 AM
OR straight away culture the thawed cells from the cryovial in a culture flask ?
Posted 14 September 2012 - 04:49 PM
Posted 19 September 2012 - 10:36 PM
Once I worked with ATCC cells (keratinocytes primary cells), and even though it was stored with 10% DMSO, but ATCC protocol said I should culture it directly after thawing no centrifuge, then remove the older medium with remaining of DMSO after 24 hrs of culturing.