Difficulty in cutting tissue after collection of sample in RNAlater
Posted 14 September 2012 - 01:29 AM
The samples were kept overnight in 4 degrees, and transferred to -80 degrees the next day.
When trying to cut the tissue samples with cryostat,
the samples seem to split and break and I cannot prepare my slides with this samples.
Before subjecting my samples to the OCT medium, I just dab the samples on a tissue paper to get rid of the RNAlater.
So do the difficulty in cutting the tissue samples are caused by RNAlater ?
How to remove the RNAlater covering the tissue sample effectively before subjecting them for slide preparation ?
Hope that someone out there can help me to overcome this problem.
Posted 14 September 2012 - 08:13 PM
Posted 14 September 2012 - 09:46 PM
Posted 16 September 2012 - 06:12 AM
I have just came across an article that mentioned to incubate the tissue collected in RNAlater in ice-cold PBS for 5-10mins before cutting them using cryostat.