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#1 anonymous



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Posted 02 May 2001 - 09:00 PM

i am trying to express a cloned gene in mammalian cells.i have tried 3 different cell lines and two different methods also.one is calcium chloride and the other is QIAGEN trasfection reagent EFFECTENE.after trasfection i am doing a western to confirm the presence of protien but i am not getting any signal..toghether with this i am using a GFP vector as a reporter.there also i can see only very few cells expressing GFP.can anybody out there give me any suggestions?

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