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PCR calculation


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#1 calculation

calculation

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Posted 12 September 2012 - 12:54 PM

Another stupid question, but please help:

i want to have a 25 uL PCR reaction.
i have 22uL of master mix 2 uL of forward and reverse primers

I have a DNA of stock concentration of 165 ng/uL but for my PCR reaction i need 8 ng of DNA, so how much from the stock should i take and add to the PCR reaction mix?

#2 bob1

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Posted 12 September 2012 - 12:58 PM

have a look at any one of the many many dilution calculation threads on here. There is an excellent one pinned at the top of the subforum you posted in.




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