6 replies to this topic

[calculation]

What do you mean by 1:10000 X dilution?

I have 5 uL of gel red and i need to dilute it to 1:10000X before use.

I know its a silly question but Can some one help? Also if you could tell me how to calculate then it would be very nice of you.

[siddharthsameer]

calculation, on 12 September 2012 - 03:49 AM, said:

What do you mean by 1:10000 X dilution?

I have 5 uL of gel red and i need to dilute it to 1:10000X before use.

I know its a silly question but Can some one help? Also if you could tell me how to calculate then it would be very nice of you.

i think you can dilute 1:10 four times each from the previously made  and then u can achieve it

[leelee]

1:10000 usually means 1 part to 10000 parts. So in your case 1 part gel red to 10000 parts molten agarose.

Some people also use it to mean a dilution of 1 in 10000 (so 1 part gel red to 9999 parts agarose).

For your application I would add it neat to your molten gel (so if you are making a 50ml gel, add 5ul), and not dilute it first.

Actually, I would suggest using it at 1 in 40 000, which is what we do and we still get beautiful bright bands (with about 1/8th of a second exposure time for photos).

[calculation]

@Lelle - I add it to the molten gel.

But don't i need it to dilute it in water first and then start using it?

So, how much should i add if i am taking it in 1:40000

(can you tell me how do you calculate it?)

Edited by calculation, 12 September 2012 - 06:23 AM.

[leelee]

Nope, you use it at 1 in 10 000 so essentially you are diluting it in your molten gel.

[calculation]

So, how much should i add if i am taking it in 1:40000

(can you tell me how do you calculate it?)

[leelee]

This is how I work it out:

Dilution factor is 40 000, volume of gel is, for example, 50ml

50ml/ 40 000= 0.00125ml (then x 1000 to give the volume in ul, so 1.25ul)