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Quantification of density of DNA bands


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#1 nostaljikkedi

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Posted 27 October 2003 - 12:33 AM

[COLOR=blue]Could you please inform me about quantifying the DNA bands that I got from gel electrophoresis. I have a gel documentation system that I can take the pictures of the band under UV light. Now, I am trying to quantify the density of the bands. I have a software program for that on pc, but the manual is very confusing. If anybody knows and tells it in a simple way or if there is some information sites, I would be very glad to learn.

Especially, I can not understand the noise substraction part.

Thank you!

Secil CERTEL
Biotechnology and Bioengineering

#2 pcrman

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Posted 27 October 2003 - 09:30 AM

I know there are many such software even photoshop can do the job. Each software differs in terms of how to use it. I have been using one called Gelquant which is pretty easy to use. So there is no general instructions on how to quantitate your bands, just read thoroughly the help file provided by your software. :blink:

#3 nostaljikkedi

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Posted 28 October 2003 - 05:10 AM

Thank you pcrman, I have a program and manual of it but it is very confusing? what is the simpler one that you are using?

#4 pcrman

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Posted 28 October 2003 - 09:50 AM

The one I used is Gelquant or Gel-Lab found at here

http://www.ampl.com.au/gq_main.htm




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