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synchronize growth

yeast synchronize cultur

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#1 uday

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Posted 11 September 2012 - 11:57 AM

How can I get a synchronize growth of yeasts in lab ? what would be the parameters for monitoring ?

#2 El Crazy Xabi

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Excellent

Posted 11 September 2012 - 04:23 PM

You need different compounds to make them to stop at some point of the cell cycle, incubate to allow them to reach that point and then wash to remove the reactive to restart the cycle from that point:

Here, authors use some:
http://www.plosone.o...al.pone.0002223
Materials and methods --> Cell Synchrony and Fluorescence-activated Cell Sorter (FACS) Analysis

#3 Phil Geis

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Good

Posted 03 October 2012 - 06:47 PM

Here's a nice article discussing your subject.

http://www.ncbi.nlm....pubmed/22262285




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