2 replies to this topic

[uday]

How can I get a synchronize growth of yeasts in lab ? what would be the parameters for monitoring ?

[El Crazy Xabi]

You need different compounds to make them to stop at some point of the cell cycle, incubate to allow them to reach that point and then wash to remove the reactive to restart the cycle from that point:

Here, authors use some:
http://www.plosone.o...al.pone.0002223
Materials and methods --> Cell Synchrony and Fluorescence-activated Cell Sorter (FACS) Analysis

[Phil Geis]

Here's a nice article discussing your subject.

http://www.ncbi.nlm....pubmed/22262285