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Bacteria killing: I need a reversable bacteriostatic

bacteria bacteriostatic killing plating. viable

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#1 achillebro

achillebro

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Posted 11 September 2012 - 01:54 AM

hi guys,

I'm planning to do a bacteria killing test in vitro.
Basically I want to E.coli with neutrophils and see what happens. Since I'm interested in late effects I do need a bacteriostatic that allows me to maintain a constant M.O.I. and also to plate the bacteria and count the CFU the next day.

I tried with gentamycin but I had problems, i.e. no colonies on the plate.

Anyone? Many thanks





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