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designing methylation specific primers


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3 replies to this topic

#1 shelleyanne1983

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Posted 11 September 2012 - 12:51 AM

Hi! i am really stuck and could do with some help- im trying to design methylation specific primers using methyprimer, and i input the sequence and it designs primers but also says no cpgs found- i dont get it? it is this ok or not? please forgive my ignorance i seem to be going round in circles!

#2 lmg

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Posted 11 September 2012 - 12:58 AM

Input larger up- and down-stream sequence in order to see if your region of interest is CpG rich, promoter region.

#3 shelleyanne1983

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Posted 11 September 2012 - 01:05 AM

I have copied the sequence from database, however methylprimer wont accept the whole sequence as its too large- how do find the promoter region (sorry again for ignorance!)

#4 lmg

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Posted 11 September 2012 - 01:14 AM

http://www.protocol-...+promoter +find

http://www.protocol-...+promoter +find

http://www.protocol-...find#entry59286

http://www.protocol-...+promoter +find

hope it will help,
s.




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