Posted 10 September 2012 - 10:18 PM
I bought a bottel of acrylamid on which is writen for syntehsis, is it possible to use it for electrophoresis?
or i should exchange it with acrylamid for electrophoresis?
please let me know
Posted 13 September 2012 - 10:45 PM
I normally buy ready made Bis/acrylamid from Biorad. It's a long time I haven't prepared fresh acrylamid.
Posted 14 September 2012 - 10:49 PM
the purity is 99%, for electrophoresis is > 99%.
Posted 17 September 2012 - 05:31 AM
we used to use reagent grade acrylamide, decolorize and filter. our gels ran well.
- Adrian K likes this
genius does what it must
i do what i get paid to do
Posted 19 September 2012 - 06:15 PM
Posted 03 October 2012 - 06:54 AM
You should check the conductivity which should be lower than 2,5 uS/cm.