I was using a CMV promoter plasmid to express my protein in HEK cells. I was told that CMV promoter will slowly loose its activity and my protein expression will go down. Why is that?
Thanks!
0
Why cmv promoter is only for transient expression?
Started by ivycczheng, Sep 08 2012 09:23 PM
cmv promoter protein expression
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,355 posts
Thelymitra pulchella
224
Excellent
Excellent
Posted 09 September 2012 - 01:27 PM
It's usually the case that unless there is some selective pressure on the cells to maintain the promoter in an active state (e.g. antibiotic resistance), then the inserted DNA gets methylated and/or inactivated in some manner.
