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salmonella cacl2 competency


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3 replies to this topic

#1 Inbox

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Posted 08 September 2012 - 10:40 AM

Is it possible to make Salmonella typhimurium chemically competent by CaCl2 method? what will be efficiency. Thanks.

#2 Curtis

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Posted 13 September 2012 - 10:53 PM

Good question. I just asked my colleague now, he said he often uses Salmonella in electroporation and he prepares them himself. They work well, and he says you can make them chemically competent too. They must work.

#3 Inbox

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Posted 15 September 2012 - 09:01 AM

Good question. I just asked my colleague now, he said he often uses Salmonella in electroporation and he prepares them himself. They work well, and he says you can make them chemically competent too. They must work.


Thanks for reply. Can you tell efficiency of transformation by chemical transformation (cacl2 or other methods). Is it very low or what so peoples are mostly preferring electroporation.

Edited by prabhubct, 15 September 2012 - 09:02 AM.


#4 Curtis

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Posted 16 September 2012 - 10:48 AM

I usually prepare with Invitrogen's method, using cacal2. I think most people prepare with the same method. can't complain about the efficiency, but it's better to transform larger plasmids with electroporation.




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