I have to clone a ~12 Kb fragment into an E. coli plasmid.
So far I have used a p15a ori - based vector, but the plasmid seems to be not very stable.
Does anyone knows whether p15a ori can replicate a 15Kb plasmid?
Is there any better alternative (eg a BAC?).
Many thanks for any suggestion.
Cloning large size insert into E. coli plasmid.coli plasmid large inser
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