Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cloning large size insert into E. coli plasmid.

coli plasmid large inser

  • Please log in to reply
No replies to this topic

#1 miche

miche

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 07 September 2012 - 10:25 PM

Hi everyone,

I have to clone a ~12 Kb fragment into an E. coli plasmid.

So far I have used a p15a ori - based vector, but the plasmid seems to be not very stable.

Does anyone knows whether p15a ori can replicate a 15Kb plasmid?
Is there any better alternative (eg a BAC?).

Many thanks for any suggestion.

Miche




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.