4 replies to this topic

[Surya r]

Hello,

I have extracted protein from tissue in RIPA buffer.  I have got good yeild but I understood from the Kit protocol/ company's technical support that RIPA buffer cannot be used for doing ELISA's. Could anyone please tell me a working protocol to precipitate protein out of RIPA buffer so that I could use it for ELISA's. .

Many  thanks in advance.
Surya R

[mdfenko]

you can dialyze away the problematic components of the ripa.

[Surya r]

Thank you. Could you please elaborate or send me a protocol to do it. Thanks in advance

[mdfenko]

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq

[Surya r]

thank

mdfenko, on 10 September 2012 - 08:09 AM, said:

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq

thank you