Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein precipitation from RIPA buffer to perform ELISA

ELISA RIPA buffer

  • Please log in to reply
4 replies to this topic

#1 Surya r

Surya r

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 07 September 2012 - 02:38 AM

Hello,

I have extracted protein from tissue in RIPA buffer. I have got good yeild but I understood from the Kit protocol/ company's technical support that RIPA buffer cannot be used for doing ELISA's. Could anyone please tell me a working protocol to precipitate protein out of RIPA buffer so that I could use it for ELISA's. .

Many thanks in advance.
Surya R

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 07 September 2012 - 07:59 AM

you can dialyze away the problematic components of the ripa.
talent does what it can
genius does what it must
i do what i get paid to do

#3 Surya r

Surya r

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 10 September 2012 - 01:12 AM

Thank you. Could you please elaborate or send me a protocol to do it. Thanks in advance

#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 10 September 2012 - 08:09 AM

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq
talent does what it can
genius does what it must
i do what i get paid to do

#5 Surya r

Surya r

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 12 September 2012 - 06:57 AM

thank

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq

thank you





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.