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blocking Dynabeads with BSA

Dynabead pre-block BSA

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2 replies to this topic

#1 memari

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Posted 06 September 2012 - 11:54 AM

I wonder why authurs in journals recommend pre-blocking dynabeads by BSA, while the Company of Invitrogen says that "BSA blocking may not be very successful, as surface adsorption is not promoted between the hydrophilic bead surface and the hydrophobic BSA."

https://www.invitrog...ion-FAQs.html#7


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Babak

Edited by memari, 06 September 2012 - 11:57 AM.

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Babak Memari

#2 Curtis

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Posted 12 September 2012 - 08:57 PM

I also could never fully block my beads with BSA. it was like BSA isn't enough. But what to do? I would recommend doing both and compare.

#3 memari

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Posted 13 September 2012 - 06:02 AM

This is another example that biologists works nowaday with blind eays in their lab.

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Babak Memari
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Babak Memari





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