How to introduce a mutagenesis in plant?
Posted 07 September 2012 - 06:04 AM
or i just had a quick look at this paper which might get you started http://www.iaea.org/...34405682533.pdf
Posted 12 September 2012 - 08:48 AM
Posted 20 September 2012 - 02:45 AM
I'm sorry, but that's not really my area of expertise. Normally when you're working with radioactive stuff you need a special certification, which you need to renew every 5 years or so. I don't think using a UV lamp will do much to generate mutants. From the papers I've read previously the seed treatment is quite harsh and lots of embryos actually turn out defective and die. Plus, you'll need thousands of plants to detect a successful mutant.
This is a more recent publication of mutation in tomato to obtain resistance to a parasitic plant
The method they use is irradiation by fast neutrons. Quoted from the paper, so you get a rough idea
"Seeds of M-82 were irradiated by 60 Gy fast neutrons. About 17,000 M2 plants were screened for resistance against Phelipanche under field conditions, and were allowed to self-pollinate. Approximately 133 of the screened plants were found resistant to Phelipanche, 99 of which had viable seeds."
Posted 01 October 2012 - 09:39 PM
Hope that helps