Posted 06 September 2012 - 11:15 AM
I have been trying to generate Vero cells stably expressing a Golgi protein fused to GFP or a membrane protein fused to GFP. I started 3 months ago with this and until today I do not seem to have any positive colonies! I did different transfections and after 48h I split my cells in the presence of G418 (1mg/ml). My control cells they all die within 10 days but in my transfected cells I see little colonies after this time. When I check my cells in the fuorescence microscope some of them are positive but the colonies are completely heterogeneous. When I split again the cells I loose my few positive cells....
Any idea how to improve this?
Thank you very much!!
Posted 06 September 2012 - 11:57 AM
Posted 06 September 2012 - 01:46 PM