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Strange Bioanalyzer trace


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#1 Pjunior

Pjunior

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Posted 06 September 2012 - 09:50 AM

Could anyone help?

Following fragmentation and biotin labelling of cDNA in preparation for microarray hybridisation I assess the fragmentation process using the bioanalyzer trace.

The trace normally displays a single peak after the marker with the majority of transcripts under 200nt. However lately traces have been starting to show a second peak that begins about midway /bottom of the first peak.

Has anyone any idea what this peak represents? It is also displayed as a faint band when reviewing the electrophoresis.




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