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Sanger Sequencing - Capillary Lengths


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#1 Pseudopneumo

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Posted 06 September 2012 - 07:41 AM

Dear all

We had an ABI 3130 sequence analyzer, and I was just wondering how do the different capillary lengths (36cm and 50cm) differ? Do they have anything to do with the read lengths?

Thanks

#2 mdfenko

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Posted 06 September 2012 - 11:14 AM

in general: longer runs, better resolution, more bases read (depends on running conditions).

Edited by mdfenko, 06 September 2012 - 11:16 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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