agarose gel electrophoresis
Posted 06 September 2012 - 12:18 AM
Posted 06 September 2012 - 12:41 AM
thanks i added 5µl of ethidium bromide to the running buffer but i am tensed wheether i will be able to see it since i have to cut the band for the ligation...are you sure it will be fine.. thanks for your reply..
yes, it should be fine.
Posted 06 September 2012 - 02:20 AM
Posted 06 September 2012 - 03:32 AM
It worked perfectly fine
I used to visualize my bands after migration with an immersion of the gel in a solution with a high concentration of ethidium bromide for about 30 minutes. That works fine! In the running gel, probably would be fine but I think it depends also on the concentration of your sample. So, what is the result?
Posted 06 September 2012 - 12:58 PM