Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

[Question]DNaseI in treating neonatal rat cardiomyocytes; size of the cells?

cardiomyocyte primary culture

  • Please log in to reply
No replies to this topic

#1 lydialing

lydialing

    member

  • Active Members
  • Pip
  • 9 posts
1
Neutral

Posted 04 September 2012 - 08:24 PM

Hi,

I have some questions about handling neonatal rat cardiomyocytes and I’d appreciated your input.

For those who have cultured neonatal rat cardiomyocytes, do you use DNase I before filtering the cell suspension after collagenase treatment? What’s the amount of DNaseI you used and for how long?
In addition, for people who have counted the cell surface area of these cardiomyocytes, in what size it usually is (um^2)?
The values counted from our lab members sometimes vary a lot, so I just think that data from a third-party might help us in this point.

Thank you very much.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.