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[Question]DNaseI in treating neonatal rat cardiomyocytes; size of the cells?

cardiomyocyte primary culture

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#1 lydialing



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Posted 04 September 2012 - 08:24 PM


I have some questions about handling neonatal rat cardiomyocytes and I’d appreciated your input.

For those who have cultured neonatal rat cardiomyocytes, do you use DNase I before filtering the cell suspension after collagenase treatment? What’s the amount of DNaseI you used and for how long?
In addition, for people who have counted the cell surface area of these cardiomyocytes, in what size it usually is (um^2)?
The values counted from our lab members sometimes vary a lot, so I just think that data from a third-party might help us in this point.

Thank you very much.

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