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petites in yeasts

yeast petities fermentation

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#1 uday



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Posted 04 September 2012 - 08:21 PM

Anybody work with petites (respiratory deficient mutants) of yeasts? I suddenly noticed some petite like colonies and what could be the easiest and reliable way to characterize them?

#2 Phil Geis

Phil Geis


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Posted 01 October 2012 - 07:14 PM

Saccharomyces cerevisiae petites have no or non-functional mitochondria and require an external souce of some lipids for extended growth. As I recall, petite cells do not reduce TTC (triphenyl tetrazolium) in the presence of glucose to a red color while respiratory competent cells do.

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