Anybody work with petites (respiratory deficient mutants) of yeasts? I suddenly noticed some petite like colonies and what could be the easiest and reliable way to characterize them?
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Phil Geis
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Posted 01 October 2012 - 07:14 PM
Saccharomyces cerevisiae petites have no or non-functional mitochondria and require an external souce of some lipids for extended growth. As I recall, petite cells do not reduce TTC (triphenyl tetrazolium) in the presence of glucose to a red color while respiratory competent cells do.
Also tagged with one or more of these keywords: yeast, petities, fermentation
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