HI EVERYBODY
can any one send me a simple protocol to MITOCHONDRIAL DNA extraction ? and how can i confirm after that that my DNA extracted is truly mitochondrial
thaaaanks
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6 replies to this topic
#2
hobglobin
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Posted 04 September 2012 - 11:44 AM
usually it is extracted together with genomic DNA. As there are many mitochondria in the cells you'll have sufficient in your gDNA finally with all protocols.
If you need pure mtDNA, one way would be ultra-centrifugation to get a fraction of cell organelles with mitochondria and then DNA extraction.
If you need pure mtDNA, one way would be ultra-centrifugation to get a fraction of cell organelles with mitochondria and then DNA extraction.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#3
lula
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Posted 04 September 2012 - 12:58 PM
BUT WHICH KIT IS BETTER ? OR IS SALTING OUT TECHNIQUE FOR GENOIMIC DNA IS SUFFEICIENT
#4
hobglobin
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Posted 05 September 2012 - 07:57 AM
I'd say most kits are reliable (I only used a few and did not find much differences, except the price). Salting out works too for mtDNA but the DNA is more "dirty", which is for PCRs usually no problem.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#5
lula
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Posted 05 September 2012 - 11:59 AM
by dirty u mean with organells or genomic DNA?
and how can u measure ur mitochondrial DNA amount ( purre mt DNA not geneomic)?
and how can u measure ur mitochondrial DNA amount ( purre mt DNA not geneomic)?
#6
hobglobin
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Posted 06 September 2012 - 03:56 AM
mostly proteins of course...and other stuff depending what source of DNA you have
no idea how you can measure the mtDNA amount
no idea how you can measure the mtDNA amount
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#7
lula
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Posted 08 September 2012 - 06:47 AM
thank u for ur replies
