Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ELISA quantification

4PL nonlinear regress.

  • Please log in to reply
2 replies to this topic

#1 moljul

moljul

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 130 posts
2
Neutral

Posted 04 September 2012 - 06:13 AM

how can I do ELISA quantification using the 4 parameter logistic (4PL) nonlinear regression? my plate reader is not capable to do this!

can I perform 4PL in excel or is there a different program available for this quantification strategy?

where can I get the values for the following variables: F(x) = ((A-D)/(1+((x/C)^B ))) + D

thanks in advance,
moljul

Edited by moljul, 04 September 2012 - 06:14 AM.


#2 DRT

DRT

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 160 posts
7
Neutral

Posted 04 September 2012 - 12:26 PM

Do a forum search for 'curve fitting'; there are a lot of posts for various software options.

#3 Sotirios

Sotirios

    member

  • Active Members
  • Pip
  • 23 posts
0
Neutral

Posted 07 September 2012 - 01:11 AM

Unfortunately you have to use a program like graph pad or origin
Athanasiou Sotirios




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.