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Peptide Conc @215 and 225nm?


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#1 saad

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Posted 04 September 2012 - 04:33 AM

Hi Guys,

I have a peptide with one tyrosine and no Try or Cys.
I used the nanodrop previously but it seems that the accuracy wasn't good to measure the affinity using ITC.
-I want to measure the concentration using the Absorbance at 215 nm and 225nm then use this equation: ug/ml=144*(215-225)
So I need to know the concentration by uM. How can I convert to molar concentation. The MWt of the peptide is about 3237.3 Da.
- The other question related to 215/225 I think the abo should be less than 1.5 to be accurate, is it right.

Thank you so much

#2 proteaMatt

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Posted 04 September 2012 - 12:04 PM

This might be of some help to you, I use it all the time. Promega Biomath Protein Molar Conversions Calculator
Lab Technician at Protea Biosciences




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