Acid urea gel electrophoresis (AUGE)
Posted 04 September 2012 - 01:35 AM
I am doing AU and TAU gel electrophoresis for some basic proteins by Sandra Hake Method "Extraction, purification and analysis of histones" Nature Protocols (2007). 2(6): 1445-1457.
Since the basic proteins are positively charged so they move in the opposite direction as that of SDS.
What i dont understand is that the author is asking to switch the electrode leads in Acid urea gel electrophoresis so that the positively charged proteins run towards the cathode. How should i switch the electrode leads? either the orientattion of leads going in the power supply or the electrode leads going in the tank, or there is anything else. I am using BioRad Mini Protean III apparatus. please help me i am really confused.
Posted 04 September 2012 - 02:49 AM
I thought it was quite the fun to reverse the current.... Good luck!
Posted 04 September 2012 - 10:08 AM
Posted 15 January 2013 - 01:35 AM
Posted 15 January 2013 - 11:15 AM
genius does what it must
i do what i get paid to do
Posted 16 January 2013 - 02:50 AM
is cytochrome c the only maker of AU gel?
Posted 16 January 2013 - 02:54 AM
Posted 16 January 2013 - 03:11 AM