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Acid urea gel electrophoresis (AUGE)


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7 replies to this topic

#1 pakbiochemist

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Posted 04 September 2012 - 01:35 AM

Dear friends,

I am doing AU and TAU gel electrophoresis for some basic proteins by Sandra Hake Method "Extraction, purification and analysis of histones" Nature Protocols (2007). 2(6): 1445-1457.
Since the basic proteins are positively charged so they move in the opposite direction as that of SDS.

What i dont understand is that the author is asking to switch the electrode leads in Acid urea gel electrophoresis so that the positively charged proteins run towards the cathode. How should i switch the electrode leads? either the orientattion of leads going in the power supply or the electrode leads going in the tank, or there is anything else. I am using BioRad Mini Protean III apparatus. please help me i am really confused.

Regards

Binsan

#2 michele.xyz

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Posted 04 September 2012 - 02:49 AM

I used to run BAC gels which is a different type of acidic electophoresis, and all I did was I plugged the black lead into the red socket and the red one into the black socket and that worked just fine. I used a biorad apparatus too but I can't tell you what exact make they were, and they were big tanks for large gels.

I thought it was quite the fun to reverse the current.... Good luck!

#3 pakbiochemist

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Posted 04 September 2012 - 10:08 AM

oh thank you so much. you solved my problem

Regards
Binsan

#4 yongzhi tian

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Posted 15 January 2013 - 01:35 AM

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus work well for that change?

#5 mdfenko

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Posted 15 January 2013 - 11:15 AM

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus work well for that change?

yes
talent does what it can
genius does what it must
i do what i get paid to do

#6 yongzhi tian

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Posted 16 January 2013 - 02:50 AM


to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus work well for that change?

yes

thank you!
is cytochrome c the only maker of AU gel?

#7 yongzhi tian

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Posted 16 January 2013 - 02:54 AM

is cytochrome c the only maker of AU gel? or anyone else?

#8 yongzhi tian

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Posted 16 January 2013 - 03:11 AM

are there any requirements for cytochrome c used by AU gel? could you recommend the very ones for AU gels? thanks!




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