7 replies to this topic

[pakbiochemist]

Dear friends,

I am doing AU and TAU gel electrophoresis for some basic proteins by Sandra Hake Method "Extraction, purification and analysis of histones" Nature Protocols (2007). 2(6): 1445-1457.
Since the basic proteins are positively charged so they move in the opposite direction as that of SDS.

What i dont understand is that the author is asking to switch the electrode leads in Acid urea gel electrophoresis so that the positively charged proteins run towards the cathode. How should i switch the electrode leads? either the orientattion of leads going in the power supply or the electrode leads going in the tank, or there is anything else.  I am using BioRad Mini Protean III apparatus. please help me i am really confused.

Regards

Binsan

[michele.xyz]

I used to run BAC gels which is a different type of acidic electophoresis, and all I did was I plugged the black lead into the red socket and the red one into the black socket and that worked just fine. I used a biorad apparatus too but I can't tell you what exact make they were, and they were big tanks for large gels.

I thought it was quite the fun to reverse the current.... Good luck!

[pakbiochemist]

oh thank you so much. you solved my problem

Regards
Binsan

[yongzhi tian]

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus  work well for that change?

[mdfenko]

yongzhi tian, on 15 January 2013 - 01:35 AM, said:

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus  work well for that change?

yes

[yongzhi tian]

mdfenko, on 15 January 2013 - 11:15 AM, said:

yongzhi tian, on 15 January 2013 - 01:35 AM, said:

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus  work well for that change?

yes

thank you!
is cytochrome c the only maker of AU gel?

[yongzhi tian]

is cytochrome c the only maker of AU gel?  or anyone else?

[yongzhi tian]

are there any requirements for cytochrome c used by AU gel? could you recommend the very ones for AU gels? thanks!