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Cleaning Glassware using Nitric Acid

Glassware

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5 replies to this topic

#1 Smog187

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Posted 03 September 2012 - 11:32 AM

Hello everyone,

Does anyone out there have any information for cleaning glassware using Nitric Acid? Any other methods to clean glassware are appreciated.

Thanks!

#2 taasmore

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Posted 03 September 2012 - 12:14 PM

Iam having similar proble. My conicals got contaminated and autoclaving did not solve the problem. My guide directed me to use nitric acid or HCl over night soaking of the glassware but feeling ashamed to ask him the concentration to use and how to use it.

#3 phage434

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Posted 03 September 2012 - 12:29 PM

It depends a lot on what you are cleaning and how clean you must get the glassware. If it is DNA, then soaking overnight in 1 M HCl will easily do the trick. If you need "no organics present" clean, then usually pirhana (mixture of concentrated H2SO4 and 30% hydrogen peroxide) is used. Not for the faint hearted or careless.

#4 mdfenko

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Posted 03 September 2012 - 02:34 PM

i used to soak quartz cuvettes in concentrated nitric acid.

other soaks are:

chromic acid (not recommended anymore)

nochromemix (replacement for chromic acid)

both prepared in concentrated sulfuric acid

various detergents (eg alconox, tergazyme, micro, etc)

depends on what you are cleaning off of your glassware.

Edited by mdfenko, 03 September 2012 - 02:35 PM.

talent does what it can
genius does what it must
i do what i get paid to do

#5 El Crazy Xabi

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Posted 03 September 2012 - 04:37 PM

Cleaning for trace metals, for samples wich will be analized by ICP 10% nitric acid overnight is enough, but depends on your contamination.

#6 Akdor

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Posted 05 September 2012 - 12:35 PM

You could try with Chromic Solution, it only would take few minutes, and it is not so dangerous as the piranha solution which I only recommend you in very extreme cases.




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