Southern blottingsouthern transfer
2 replies to this topic
Posted 03 September 2012 - 06:42 AM
Is there any effect from the presence of EtBr in the agarose gel for the subsequent capillary transfer for Southern blotting protocol ? I usually do EtBr staining after electrophoresis to confirm the restriction digestion of DNA samples.
Posted 03 September 2012 - 12:47 PM
It doesn't affect the transfer usually. I have always stained my Southern blots with EtBr prior to transfer and never had a problem. It may slightly retard the blotting step, but with the times (overnight) usually used for this procedure, it shouldn't be a problem.