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Southern blotting

southern transfer

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#1 uday

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Posted 03 September 2012 - 06:42 AM

Is there any effect from the presence of EtBr in the agarose gel for the subsequent capillary transfer for Southern blotting protocol ? I usually do EtBr staining after electrophoresis to confirm the restriction digestion of DNA samples.

#2 bob1

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Posted 03 September 2012 - 12:47 PM

It doesn't affect the transfer usually. I have always stained my Southern blots with EtBr prior to transfer and never had a problem. It may slightly retard the blotting step, but with the times (overnight) usually used for this procedure, it shouldn't be a problem.

#3 uday

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Posted 04 September 2012 - 08:11 PM

Thanks a lot !




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