4 replies to this topic

[pppyeah]

hi,

I do blunt end ligation over night by seting the temp 12°C as it should be 16°C。

and now i increase the temp to 16°C.

did this makes any different to the result?

thanks thanks

[prabhubct]

You may get colonies but may beless ligation efficiency.

[pppyeah]

prabhubct, on 03 September 2012 - 02:26 AM, said:

You may get colonies but may beless ligation efficiency.

thanks, can i do something now to improve?
MY plan is keep it 16 for another 5 hour

[prabhubct]

No I don't think there is much you can do when temperature is not set at desired. But Don't worry you should get colonies at 12 ⁰C incubation too.
People usually do 4 ⁰C overnight incubation. Its may be better you directly try transformation now.

[pppyeah]

prabhubct, on 03 September 2012 - 03:44 AM, said:

No I don't think there is much you can do when temperature is not set at desired. But Don't worry you should get colonies at 12 ⁰C incubation too.
People usually do 4 ⁰C overnight incubation. Its may be better you directly try transformation now.

actually i am not doing transformation,this is just a step in my microarray preparation.

thanks