Question about HeLa cell overconfluence
Posted 31 August 2012 - 05:38 PM
Would appreciate input on what is probably a really basic question. Our lab has been using HeLa cell-based lines. There have been a couple of times when I've unfortunately allowed them to become confluent. After I split them, they still seem to grow fine. However, I have one labmate who says that whenever that happens, I should throw them out and start with a fresh vial, while another labmate says that whatever confluence-induced changes occur will be washed out after a couple of passages. Which one of them is right?
Posted 01 September 2012 - 01:31 PM
Posted 05 September 2012 - 12:39 PM