
What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely
#1
Posted 31 August 2012 - 05:22 AM
#2
Posted 31 August 2012 - 08:33 AM
Assuming this is a restriction enzyme digest, an easier solution would be to add another enzyme to cut the fragment you don't need into two smaller fragments, so you can more easily separate them from your band of interest.
then heaven will be yours, before you meet your end
#3
Posted 31 August 2012 - 09:08 AM
High Resolution Agarose, 0,8 - 1%
Lithium borate or Lithium acetate buffer
running distance as long as possible
not long running times (buffers allow higher voltages), e.g. 20-30V/cm
In BioTechniques (2004) 37:598-602 (Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media) you find some more informations how to do it, though you might need to do some adaptation.
- ascacioc likes this
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#4
Posted 31 August 2012 - 10:17 AM
#5
Posted 31 August 2012 - 10:56 AM
Edited by prabhubct, 31 August 2012 - 10:59 AM.
#6
Posted 31 August 2012 - 11:31 AM
#7
Posted 04 September 2012 - 05:16 AM
Hi I just Digested 2680bp with restriction site present in it but not in 2600bp. This approach is Good. Thanks for other approaches too.
#8
Posted 04 September 2012 - 05:32 AM
