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[redorange]

I want to examine the contraction of rat aorta using a tissue bath system in the presence of specific drugs. My question is how long (days or hrs) before the rat aorta becomes unresponsive to drugs and becomes unuseable for experimentation? I found several sites that can overnight ship the rat aorta with ice packs. Or do I need to go through the hassle of euthinization and dissection?

thank you

Edited by redorange, 30 August 2012 - 06:39 PM.

[prabhubct]

I know about Pig coronory you can keep it alive for 3-4 days. for rat you check it, it may be 2-3 days. usually store at 4 degree.  Any ways once its outside it continuously decreases its activity. I think you have to go through euthinization and dissection every 2-3 days. I guess more BMR animals may be reasonable reason rat aorta must be dying soon.I don't recommend it for sure you have to standardize protocol or search more.

Edited by prabhubct, 31 August 2012 - 07:12 AM.