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myc-tagged proteins

Protein interactions myc tag

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#1 Smog187

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Posted 30 August 2012 - 03:25 PM

I'm trying to decide whether or not to include a myc tag on my protein. We want to do protein binding studies, but will the myc tag cause a problem?

#2 bob1

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Posted 30 August 2012 - 05:45 PM

There's no way to tell other than to make the tag and compare to an untagged or differently tagged version. I have found that tags interfere with the localization of my current protein of interest.




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