Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Smear on agarose gel from QIAGEN extracted plant samples

smear QIAGEN agarose

  • Please log in to reply
1 reply to this topic

#1 jerrybreak



  • Members
  • Pip
  • 1 posts

Posted 29 August 2012 - 08:58 PM

Would anyone know if it is common to observe these types of smears on an agarose gel after extracting DNA using a QIAGEN DNeasy 96 kit and running 500 ng of DNA in a 1% gel at 70 volts for 3 hours inside a 4 Celsius fridge (also tried at room temperature)?

I'm planning on sending these samples for Genotyping-by-Sequencing but the requirements for such procedure include no smearing on agarose gel neither discrete low molecular weight bands.... which I think I have. Any imput will be appreciated

Attached Thumbnails

  • high-mol-DNA.jpg

#2 Curtis


    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,186 posts

Posted 29 August 2012 - 09:04 PM

I am not a plant biologist but we usually get this type of smear when we don't add RNAse in the last step of our extraction.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.