I have found the e-gel opener that is provided by invitrogen to be very difficult to use in cracking open the precast E-gels. Does anyone have any tips on how to crack it open without it? Or any other gels which are easy to open that are compatible with the E-gel base?
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phage434
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Posted 29 August 2012 - 01:34 PM
It's a pain. I'd recommend avoiding the situations where you are tempted to do it by designing your cloning strategies to eliminate the need for gel purification.
