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ChiP assay band in negative control

Chip Mouse IgG PCR

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#1 ivap

ivap

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Posted 29 August 2012 - 12:46 AM

Hello there,

I have been doing ChiP assay using a kit from Upstate, millipore.
I completed the entire procedure and did PCR for GAPDH promoter sequence with positive control (RNA Pol II antibody) and negative control (Mouse IgG).
Surprisingly i got a band in both positive and negative Posted Image . The band was fainter in negative, but then there was a distinct band after PCR.

Any help on what i should do right???

ivapPosted Image





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