The general DNA extraction procedure is: lysozyme-->protease K-->cell lysis-->protein precipitation-->centrifugation-->Isopropanol DNA extraction--> rinse with 70% Ethanol-->dry-->resolve with 10mM Tris (pH8.0).
The result turns that, the A260/280 is ~1.8. But the A260/230 is very low, ~0.9-1.4. The peak at 220 is high (attached).
I tried DNA precipitation with Isopronanol to get rid of salt, and resuspend in Tris, but the A260/230 is still low.
Then, I use sodium acetate+Ethanol to get DNA precipitaiton, and resuspend in Tris, but the A260/230 is still low.
My questions are:
1. Will it interfere with qPCR efficiency? Do I need to resolve the low A260/230?
2. Would you please give me some suggestions how to resolve this problem?
Thanks a lot!
Edited by joy123, 27 August 2012 - 06:25 AM.