Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
* * * * * 1 votes

What is a use of ΔCt and ΔΔCt in qRT PCR?


  • Please log in to reply
4 replies to this topic

#1 Chinnaraja C

Chinnaraja C

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 27 August 2012 - 02:47 AM

Guys i want to start qRT-PCR using absolute quantification method to find the exact targets present in my Sample. In that i did not clear about ΔCt and ΔΔCt? Please answer me the use of ΔCt and ΔΔCt in qRT PCR?

#2 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 326 posts
21
Excellent

Posted 27 August 2012 - 11:40 AM

If you are doing Realtime PCR to check Expression level. You always need to normalize your test reading with something that constitutively express in cells. Here may be GAPDH or beta- actin as constitutively expressing genes. So You have target now and GAPDH. You determine how much is expression level of your Target gene increased or decreased during experiment. Here it suggest that you have to have control where don't influence its expression level.


So here is how much your gene expressed,

Fold Change = 2-Δ(ΔCT)

where ΔCT = CT, target - CT, GAPDH
and Δ(ΔCT) = ΔCT, stimulated - ΔCT, control

#3 zodiac1505

zodiac1505

    Enthusiast

  • Moderators
  • PipPipPipPipPip
  • 92 posts
3
Neutral

Posted 27 August 2012 - 11:40 AM

http://www.ebiometho...ods/realtimepcr

Check this link

#4 Chinnaraja C

Chinnaraja C

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 28 August 2012 - 01:34 AM

Thank you friends...Its really informative...

#5 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,198 posts
109
Excellent

Posted 31 August 2012 - 04:59 AM

In absolute quantification you use neither of these. You calculate it from the standard curve (can be found as standard curve method).

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.