Posted 27 August 2012 - 11:40 AM
If you are doing Realtime PCR to check Expression level. You always need to normalize your test reading with something that constitutively express in cells. Here may be GAPDH or beta- actin as constitutively expressing genes. So You have target now and GAPDH. You determine how much is expression level of your Target gene increased or decreased during experiment. Here it suggest that you have to have control where don't influence its expression level.
So here is how much your gene expressed,
Fold Change = 2-Δ(ΔCT)
where ΔCT = CT, target - CT, GAPDH
and Δ(ΔCT) = ΔCT, stimulated - ΔCT, control
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