Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Zebrafish RNA injection help needed


  • Please log in to reply
No replies to this topic

#1 Burtdog

Burtdog

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 23 August 2012 - 02:27 PM

Hi,

We are trying to injection Venus RNA into zebrafish embryos and so far we have had no luck. I am certain we are making RNA correctly and we have been trying to inject at 50picomol total concentration. Has anyone run into any issues injecting Venus and having expression? Are we injecting at too low a concentration? We have used the kit that we make the Venus RNA from with other constructs and they work just fine, so it seems unlikely to be the kit. We have also tried several different Venus constructs with no luck. We have ruled out the injection buffer, water and everything else in common. Any suggestions would be great. Thanks!

Victoria




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.