2 replies to this topic

[wdyeo]

Dear all,

Can we still able to extract DNA from snap freeze tissues (lungs, pancreas) of mice {snap freeze was done by using pentane in liquid nitrogen}?
May i know the best conventional method to extract DNA from these frozen tissues?
Thank you very much

wdyeo

Edited by wdyeo, 21 August 2012 - 01:59 PM.

[bob1]

Yes you can.

Any one of the conventional methods will work well, it all depends on the purity you require.  For most applications a standard phenol-chlorofom extraction is good.

[wdyeo]

Dear bob1,
Thanks alot for the reply
Have a nice day