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can we extract DNA from snap freeze tissue


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#1 wdyeo

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Posted 21 August 2012 - 01:56 PM

Dear all,

Can we still able to extract DNA from snap freeze tissues (lungs, pancreas) of mice {snap freeze was done by using pentane in liquid nitrogen}?
May i know the best conventional method to extract DNA from these frozen tissues?
Thank you very much Posted Image

wdyeo

Edited by wdyeo, 21 August 2012 - 01:59 PM.


#2 bob1

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Posted 21 August 2012 - 05:44 PM

Yes you can.

Any one of the conventional methods will work well, it all depends on the purity you require. For most applications a standard phenol-chlorofom extraction is good.

#3 wdyeo

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Posted 22 August 2012 - 01:22 PM

Dear bob1,
Thanks alot for the reply
Have a nice day :D




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