We have been running our PCR products in 1.2% agarose gels in 1XTAE buffer using post-stain SYBR gold. SYBR gold is supposed to be higher in sensitivity for detection; however, we have been experiencing a lot of problems with visualizing clear bands. The bands come out extremely blurry, including the DNA ladders. I have contacted the company for SYBR gold and have had no good luck. Has anyone else experienced this? Can anyone give me advice?
Thank you.
Submit your paper to J Biol Methods today!
SYBR gold post-stain makes blurry gels
Started by clarashin1813, Aug 21 2012 10:47 AM
SYBR gold blurry post-stain gel agarose troubleshooting
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 5,904 posts
Thelymitra pulchella
425
Excellent
Excellent
Posted 21 August 2012 - 12:42 PM
How long are you staining for? We may need a complete gel run and stain protocol to be able to diagnose the problem...
Also tagged with one or more of these keywords: SYBR gold, blurry, post-stain, gel agarose, troubleshooting
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Non especific band in PCR...Started by lootches, 26 Dec 2014  PCR, ANEALING, TROUBLESHOOTING
|
|
|
||
Protocols and Techniques Forums →
Cell Biology →
Cells are unhealthyStarted by Jingchun, 13 Mar 2014 cell culture, troubleshooting
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Qubit Standard 2 Green RFU value variationStarted by David Gray Lassiter, 04 Sep 2013 Qubit, DNA, Assay and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Immunology →
ELISA and Immunoassay →
Elusive protein refuses to be found in human serum with ELISAStarted by More of the Same 66, 24 Jun 2013 ELISA, Troubleshooting, Serum and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Stem Cell →
Improving yield of embryoid bodies from AggreWell plates?Started by sschaef, 29 May 2013 stem cells, embryoid bodies and 3 more...
|
|
|
