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SYBR gold post-stain makes blurry gels

SYBR gold blurry post-stain gel agarose troubleshooting

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#1 clarashin1813



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Posted 21 August 2012 - 10:47 AM

We have been running our PCR products in 1.2% agarose gels in 1XTAE buffer using post-stain SYBR gold. SYBR gold is supposed to be higher in sensitivity for detection; however, we have been experiencing a lot of problems with visualizing clear bands. The bands come out extremely blurry, including the DNA ladders. I have contacted the company for SYBR gold and have had no good luck. Has anyone else experienced this? Can anyone give me advice?

Thank you.

#2 bob1


    Thelymitra pulchella

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Posted 21 August 2012 - 12:42 PM

How long are you staining for? We may need a complete gel run and stain protocol to be able to diagnose the problem...

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