We have been running our PCR products in 1.2% agarose gels in 1XTAE buffer using post-stain SYBR gold. SYBR gold is supposed to be higher in sensitivity for detection; however, we have been experiencing a lot of problems with visualizing clear bands. The bands come out extremely blurry, including the DNA ladders. I have contacted the company for SYBR gold and have had no good luck. Has anyone else experienced this? Can anyone give me advice?
Thank you.
Submit your paper to J Biol Methods today!
SYBR gold post-stain makes blurry gels
Started by clarashin1813, Aug 21 2012 10:47 AM
SYBR gold blurry post-stain gel agarose troubleshooting
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 5,115 posts
Thelymitra pulchella
327
Excellent
Excellent
Posted 21 August 2012 - 12:42 PM
How long are you staining for? We may need a complete gel run and stain protocol to be able to diagnose the problem...
