Posted 21 August 2012 - 07:25 AM
I have been culturing MDA-MB-231 cells for the past couple of months on DMEM+10% FBS. I got these cells from another lab but I have no idea about the passage number. When I got the cells from them I was told not to worry about the passage number as these are cancer cells and they will continuously keep growing. Initially when I was culturing these cells they were fine and I had no issues.
However for the last few passages I have observed that I am getting an increasing number of senescent cells i.e. first there were one or two per plate but as I am passaging them they seem to increase in number-- see attached pics.Has anyone had a similar problem? Should I just go ahead and buy a new batch of cells???
Posted 21 August 2012 - 12:48 PM
Posted 21 August 2012 - 08:02 PM