17 replies to this topic

[prabhubct]

ascacioc, on 23 August 2012 - 01:51 PM, said:

I did a PCR once with primers one at 46 and the other one at 71 (long story, but I couldn't design them better no matter what I did, and I struggled quite a lot). Worked perfectly....but I'm the one with a too complicated PCR program

Andreea

did you got some amplification ? How did you do that?

[ascacioc]

see my previous posts, after I said that I did that I also posted a protocol I use for Phusion PCR.

Andreea

[Trof]

Open source is a nice thing and surely if someones digs out the right composition by trying and googling good for him, on the other hand I have no problem with paying company for a good product they developed